2025Year

Second Semester

Advanced Cell Technology

Lecture

Advanced Cell Technology

Z1900002 Advanced Cell Technology

IMAMURA Yasutada

2.0Credits

Fri.1Period

Hachioji Remote

A) A high degree of specialized expertise　80%
B) The skills to use science and technology　0%
C) The ability to conduct research independently, knowledge pertaining to society and occupations, and sense of ethics required of engineers and researchers　0%
D) Creative skills in specific areas of specialization　20%

This is a graduate-level cell technology course. The course provides the knowledge about basic aspects of interactions between animal cells and their surrounding environments called extracellular matrices (ECM). The matrixes, especially collagen matrixes, work as a kind of solid phase in the body and their long-term effects on cells are related to biological phenomena over a relatively long time. A synthetic way of thinking how the cells and the extracellular matrixes could be integrated into diseases or health in human is suggested.

On completion of this course, the student should be able to:
• Have an awareness of basic knowledge related collagen molecules and their aggregates.
• Have an awareness of the structure of basement membrane and their role in cell proliferation and differentiation.
• Have an awareness of ideas of tissue organization
• Have an awareness of basic ideas “iterative interaction’ and ‘internal medical approach to tissue regeneration’.
• Demonstrate the synthetic way of thinking.

The prerequisite is that you are interested in the extracellular matrix and have already begun research.

Discussion Debate／Support for self-learning using ICT／Not used

Subjects in the course:
1. Science, chemistry, and engineering in collagen research
2. Introduction to collagen
(1) Genome structure
More than 50 exons, 54 bp
　 A case of Ehlers-Danlos Syndrome: Splicing defects in type V collagen
(2) The unique feature of Amino acids composition of fibril forming collagens
(3) Biosynthetic processes
Hydroxylation, glycation, PPI reaction, PDI reaction, processing, cross-linking
(4) Effect of metabolites on the biosynthesis
2-oxoglutarate, O2, Ascorbate
3. Purification method
(1) Extraction and salt fractionation
(2) Purification with various chromatography methods
Column chromatography
Ion exchange-, Affinity-, Reverse Phase-
(3) Analysis
SDS-PAGE
Amino acid analysis, Sequence analysis, Mass spectroscopy
CD, DSC, Enzyme digestion
(4) A case of the purification of type V collagen subtypes with heparin affinity column chromatography
4. Fibril formation
Model of collagen association
5. Prolyl hydroxylation
(1) Requirement of ascorbate
(2) Hypoxia and HIF-1a
(3) Mutations in Isocitrate dehydrogenase
(4) Comparison of prolyl hydroxylation between collagen and HIF-1a
6. Tissue organization (1)
(1) Iterative interaction between cells and ECMs
(2) Generalized tissue model : the graded organization of collagen fibrils and the cellular localization
(3) The graded organization of collagen fibrils in horse tendon
7. Presentation and discussion about student's subject
8. Reconstitution of collagen aggregates
(1) Type V collagen forms thin fibrils
(2) A case of skin fragility in a cattle similar to Ehlers-Danlos Syndrome in human
9. Interaction between cells and collagens
(1) Effect of endogenously produced collagens on the cell : Ascorbate 2-phosphate enhances the production.
(2) Effect of exogenously added collagens on the cell
Type I collagen gel : in-gel culture or on-gel culture
(3) Dermis model with cell culture in contracted collagen gel
10. Reconstituted collagen aggregates as cell culture substrates
(1) Type V collagen fibril
(2) Basement membrane-like structure of type IV collagen aggregates
11. Basement membrane
(1) Structure of basement membrane and its components
(2) Basement membrane is important for proliferation and differentiation
(3) Artificial alveolus on basement membrane
12. Stem cell and basement membrane
(1) ES cells and iPS cells
(2) Laminin 511 and stem cells
(3) Regenerative medicine and cell sheet technology
13. Modification of basement membrane and possible internal medical approach to tissue regeneration
(1) Production of non-triple helical collagen polypeptides of type IV collagen (NTH aIV)s) under the ascorbate depleted condition
(2) Possible role of NTH a(IV)s
14. Reviewing of the course

Students must submit a report at the end of term about a synthetic way of thinking in their own research fields. In the report, basic ideas based on the way, such as “iterative interaction’ and ‘internal medical approach to tissue regeneration’ in the course, should be presented.

Direct feedback will be provided via email, chat, or in-person.

授業の中で指示する。

Friday, PM4 - 6, Build 17 Room 355

Not applicable

Applied Chemistry and Chemical Engineering Program